DKFZ / Chirurgische Klinik
Eintrag vom 25.01.2021
|Für Studierende der:||Medizin, life sciences|
|voraussichtliche Dauer:||12 Monate|
|Abteilung:||Translationale Chirurgische Onkologie (A430)|
|Doktorvater / -mutter:||Prof. Dr. Sebastian Schölch|
|Betreuer:||Prof. Dr. Sebastian Schölch / Lei Zhu|
Beschreibung:ARID1A protein is part of the SWI/SNF complex, which plays a crucial role in Chromatin remodeling. Our group has generated mouse lines with pancreas-specific conditional mutations in different oncogenes including Arid1a in order to develop a model for Arid1a-deficient pancreatic ductal adenocarcinoma. Suprisingly, we found that conditional loss of ARID1A in the developing murine pancreas results in postnatal lethality.
Therefore, this dissertation project will aim at characterizing the pancreatic Arid1a-deficient mouse model in order to explore the mechanism leading to this phenotype. Literature suggests a role of ARID1A in the development of pancreatic islets; therefore we hypothesize that this mouse model fails to develop pancreatic islets, leading to early postnatal lethality.
Methods we are typically using:
- Genetically engineered mouse models (GEMM)
- Primary cell culture, 3D organoid cultures
- standard laboratory techniques
- Histology, Pathology, Immunohistochemistry/Immunofluorescence
We are looking for a highly motivated student who has a strong interest in exploring the unknown and plans to spend 6-12 months to complete his/her doctoral dissertation in the Schölch laboratory. Experience in laboratory techniqes would be of definitive advantage, but is not a prerequisite.
This project aims at medical students with genuine interest in biomedical research which goes beyond simply obtaining a doctoral degree.
We are a young and dynamic group of international clinical and preclinical scientists. We are part of the DKFZ Heidelberg, the largest biomedical research institute in Germany. We therefore have access to a wide range of start-of-the-art core facilities and techniques. Our working language is English. Dedicated research time of all involved scientists guarantees close mentoring and training.
The laboratory and mouse house are on the Mannheim Medical Campus, therefore there is no commute to Heidelberg required.